Linear extrapolation method of analyzing solvent denaturation curves.

نویسندگان

  • C N Pace
  • K L Shaw
چکیده

The two most common methods of measuring the conformational stability of a protein are differential scanning calorimetry and an analysis of solvent denaturation curves by using the linear extrapolation method. In this article, we trace the history of the linear extrapolation method, review how the method is used to measure protein stability, and then discuss some of the other important uses.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Modeling of temperature in friction stir welding of duplex stainless steel using multivariate lagrangian methods, linear extrapolation and multiple linear regression

In this study, the temperature in friction stir welding of duplex stainless steel has been investigated. At first, temperature estimation was modeled and estimated at different distances from the center of the stir zone by the multivariate Lagrangian function. Then, the linear extrapolation method and multiple linear regression method were used to estimate the temperature outside the range and ...

متن کامل

Modeling of temperature in friction stir welding of duplex stainless steel using multivariate lagrangian methods, linear extrapolation and multiple linear regression

In this study, the temperature in friction stir welding of duplex stainless steel has been investigated. At first, temperature estimation was modeled and estimated at different distances from the center of the stir zone by the multivariate Lagrangian function. Then, the linear extrapolation method and multiple linear regression method were used to estimate the temperature outside the range and ...

متن کامل

Comparative analysis of chemical and thermal denatured 13-lactoglobu1in A in the presence of sugar osmolytes

Chemical denaturation and thermal denaturation of13-lactoglobulin A (f3 — lgA) in the absenceand presence of various concentrations sugar osmolytes and polyols were measured bymonitoring changes in the absorption coefficients at pH 2.0. It has been observed that AGD°(H20), (Gibbs free energy change in absence of denaturant at 25 °C) of f3-1gA in the presenceof 10% (w/v) Trehalose, Sucrose, Sorb...

متن کامل

Stability of proteins in the presence of polyols estimated from their guanidinium chloride-induced transition curves at different pH values and 25 degrees C.

We have recently concluded from the heat-induced denaturation studies that polyols do not affect deltaG(D) degrees (the Gibbs free energy change (deltaG(D)) at 25 degrees C) of ribonuclease-A and lysozyme at physiological pH and temperature, and their stabilizing effect increases with decrease in pH. Since the estimation of deltaG(D) degrees of proteins from heat-induced denaturation curves req...

متن کامل

A floating potential method for measuring ion density

A simple method for analyzing Langmuir probe curves is described which yields the ion density in weakly collisional plasmas for which purely collisionless theories give erroneous results. The method is based on an extrapolation to the floating potential of the saturation ion current raised to the 4/3 power. This procedure is not supported by theory but apparently works because effects neglected...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Proteins

دوره Suppl 4  شماره 

صفحات  -

تاریخ انتشار 2000